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Megazyme have recently released a new product, E-CELTH. High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Find out more about the product here. To order this kit or any other Megazyme products, please get in touch with Food Tech Solutions today.
Megazyme have recently released a new enzyme kit Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method). The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase)/lichenase (EC 22.214.171.124) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Find out more about the prodcut here. To order this kit or any other Megazyme products, please get in touch with Food Tech Solutions today.
17th December 2015: NEW PRODUCT: K-TDFR-100A & K-TDFR-200A Total Dietary Fibre
Megazyme have recently released a smaller version of the total dietary fibre kit (K-TDFR), splitting it into a large (200 assay) and small (100 assay), to offer greater flexibility and availability to customers.
This shake up of their existing TDF kit should be of advantage for those wishing to test TDF without having large sample volumes.
Find out more here
16th June 2015: NEW PRODUCT: E-PROTOF - Protoplast F
Enzyme mix for the preparation of protoplasts from Aspergillus niger used in the biofuel, fermentation and research industry. The mix is in a ready to use format and requires no manipulation prior to use. It is capable of producing protoplasts from Aspergillus niger which can be used to transform fungus with foreign DNA. Optimal protoplast number generation, simple clear method, single enzyme mix, ready to use and cost effective.